Valorization of Chenopodium quinoa residues through saponin recovery: Extraction, purification, and evaluation of antioxidant and cytotoxic activities

Florez-Martinez E.; Heredia-Rondón O.; Choquenaira-Quispe C.; Buitron-Mercado M.F.; Huamán-Castilla N.L.; Tejada-Meza K.; Colina-Andrade G.D.J.; Terán-Hilares R. · Applied Food Research (2026) · DOI: 10.1016/j.afres.2026.101908

Resumen

Residues of Chenopodium quinoa , accounting for approximately 8% of the grain weight, represent an underutilized resource. In this study, saponins were extracted using hydrodynamic cavitation (HC), followed by membrane filtration, adsorption on AB-8 resin, and liquid–liquid partitioning. HC achieved an extraction yield of 66.54%, while membrane filtration increased saponin purity from 32% to 42%. Adsorption with AB-8 resin yielded a maximum purity of 65% after desorption with 70% (v/v) ethanol (CMC = 0.33 g/L), and further purification by liquid–liquid partitioning increased purity to 75%. Rutin and quercetin were identified as the predominant phenolic compounds in the purified extracts. Higher saponin purity was associated with enhanced antioxidant capacity. In addition, the extract with 65% exhibited better cytotoxic activity against MDA-MB-231, HCT-15, and A549 cancer cell lines, exhibiting low IC50 values and significantly reduced colony formation. These results demonstrate that quinoa residues are a valuable source of saponins with relevant biological activities and application potential. Copyright

Instituciones: Universidad Nacional de Moquegua; Universidad Católica de Santa María