Sperm chromatin stability and their relationship with fertilization rate in Sheep of the Junín race

Unchupaico-Payano I.; Alponte-Sierra A.; Quispe-Eulogio C.; Ancco-Goméz E.; La Cruz A.H.-D.; Mariño-Alfaro J.; Patiño-Rivera A.; Lavado-Meza C.; Huanca-Rojas L.; Bazán-Alonso L. · Revista Cientifica de la Facultad de Veterinaria (2022) · DOI: 10.52973/rcfcv-e32157

Resumen

The objective of this research was to evaluate the effect of sperm on chromatin stability and its relationship with the membrane integrity structural – physiological and the rate of fertilization of female sheep. Ejaculates of sperm (2 × 109 sperm·mL-1) with 70% of motility were collected using an artificial vagina (n=5, 2 years old. For this, each ram was served with fifteen female sheep (n=75), generating thus five different Groups (A, B, C, D, and E). A control Group also was considered. Sperm nuclear chromatin stability (NCS) was evaluated using the Borate Buffer (BB), Sodium Dodecyl Sulfate (SDS), and the mixture of Ethylenediaminetetraacetic acid (EDTA) + SDS. The fertilization rate was evaluated after 16-18 hours post sperm injection. Sperm concentration showed a significant difference (P<0.05) between Groups. In Contrast, seminal volume, and sperm motility do not show a significant difference (P>0.05). A high correlation (r2=0.52) was observed between morphology and motility, and the fertilization rate was 74.6% (n=56). It was concluded in general that techniques to evaluate nuclear condensation values do have a high likelihood to give a diagnosis about the future potential of sperm populations in Junín ram. © (2022). All Rights Reserved.

Instituciones: Universidad Peruana Los Andes; Universidad Nacional del Centro del Perú; Universidad Nacional Intercultural de la Selva Central Juan Santos Atahualpa; Universidad Peruana Unión