Single‐cell differentiation trajectories define early stages of a human cutaneous T-cell lymphoma

Cerapio J.-P.; Perrier M.; Pont F.; Laurent C.; Bertani S.; Fournie J.-J. · Exploration of Immunology (2022) · DOI: 10.37349/ei.2022.00044

Resumen

Aim: The aim of this article is to characterize in detail the γδ T lymphocytes from an adult patient with primary cutaneous T-cell lymphoma of γδ subtype (γδ CTCL). Methods: Here this article reports trajectory mapping on high-resolution differentiation trajectories of γδ T lymphocytes digitally extracted from a scRNAseq dataset. Results: In the patch-to-plaque progression of CTCL, the TCRVγnon9 subset of γδ T cells differentiated from naive T cells (Tn) and central memory T cells (Tcm) to abundant effector memory T cells (Tem) while other cutaneous γδ T and CD8 T cells remained unchanged. Conclusions: This transcriptomic switch underlies the emergence of a CTCL-like progression of the TCRVγnon9 γδ T subtype and suggests new routes for treating these diseases. © The Author(s) 2022.