RESISTANCE TO COLISTIN AND FOSFOMYCIN IN EXTENDED-SPECTRUM BETA-LACTAMASE-PRODUCING Escherichia coli ISOLATES FROM CHICKENS FOR HUMAN CONSUMPTION

Roldan-Galarreta A.P.; Arce-Servat M.F.; Arancivia-Neira F.; Gonzales-Escalante E. · Revista Peruana de Medicina Experimental y Salud Publica (2025) · DOI: 10.17843/rpmesp.2025.423.14482

Resumen

The objective of our study was to determine the resistance to colistin and fosfomycin of extended-spectrum beta-lactamase-producing Escherichia coli (Ec-BLEE). We conducted a cross-sectional descriptive study in which we analyzed 79 isolates of Ec-BLEE obtained from a previous study involving 300 chicken cloacal swab samples. We processed the isolates in the Microbiology and Immunology Laboratory of the Faculty of Medicine of the Universidad de Piura, Lima-Peru. We determined the sensitivity to colistin with the Colistin-AgarSpot detection medium and the sensitivity to fosfomycin by antibiogram according to the CLSI guidelines. We searched for the mcr-1 and fosA3 genes through PCR. We found a resistance to colistin in 17,7% and to fosfomycin in 77% of the samples. We did not detect the mcr-1 gene in the colistin-resistant isolates. The fosA3 gene was present in 100% of the fosfomycin resistant isolates.

Instituciones: Universidad de Piura