Protease and bioactive hydrolysate production by Pseudomonas sp. SU24 through bioconversion of poultry by-products

Calderón-Toledo S.; Zavaleta A.I.; Palma-Villanueva J.; Cayetano-Osorio D.; Izaguirre V.; Flores-Fernández C.N.; Brandelli A. · Revista Peruana de Biologia (2025) · DOI: 10.15381/rpb.v32i2.29929

Resumen

This study aimed to investigate protease and hydrolysate production by Pseudomonas sp. SU24, isolated from soils in Bongará province (Amazonas Department, Peru), using poultry waste as culture substrate. Enzyme production was evaluated by assessing incubation time, poultry waste concentration, and pH through a one-factor approach followed by central composite design. Production under optimized conditions was compared with skim milk and tryptic soy broth (TSB) media, while the proteolytic profile was analyzed by zymography. Biological activity prediction was performed through in silico hydrolysis of chicken feather keratin using the BIOPEP-UWM database, complemented with antioxidant activity assays (ABTS). Maximum protease production (2972.77 ± 23.89 U/mL) was achieved in the optimized medium containing 20 g/L feathers at pH 9.0 and 37°C after 96 h incubation, significantly outperforming yields in skim milk and TSB (<500 U/mL). Zymographic analysis revealed specific induction of a 55 kDa protease in the feather medium. The hydrolysates demonstrated confirmed antioxidant activity, and in silico analysis predicted a high frequency of peptides with potential ACE-I and DPP-IV inhibitory activity. This study demonstrates that Pseudomonas sp. SU24 can efficiently produce high-activity proteases and bioactive hydrolysates from poultry byproducts, representing a sustainable alternative for valorising these wastes within a circular economy framework. © Los autores.

Instituciones: Universidad Nacional Mayor de San Marcos