Potential of Streptomyces sp strains isolated from fallow soils for the control of Botrytis sp in blueberries; [Potencial das cepas de Streptomyces sp isoladas de solos em pousio para o controle de Botrytis sp em mirtilos]

Leiva-Piedra J.L.; Seclen-Pozo Y.R.; Romero-Becerra I.A. · Revista Brasileira de Meio Ambiente (2026)

Resumen

Grey mould, caused by Botrytis sp., is one of the main phytosanitary constraints in blueberry production, due to its high incidence in the field and post-harvest and the increase in isolates resistant to conventional fungicides. In this context, the present study aimed to determine the potential of strains of the genus Streptomyces spp. isolated from barren soils for the biological control of Botrytis sp. in blueberries. Strains JCH7, JCH5 and JCH3 were isolated from arid soils in the district of Puerto Eten (Peru) and characterized morphologically, biochemically and genetically. Antagonistic activity was evaluated using dual confrontation and well diffusion assays using crude extracts, compared with a commercial synthetic fungicide. The results showed inhibitions greater than 58% in dual confrontation and up to 80.98% in well diffusion, with strain JCH3 standing out for its greater anti-fungal efficacy. Biochemical tests showed the production of key hydrolytic enzymes (cellulases, lipases and proteases), as well as chitinase activity in two of the strains. Genetic identification using the 16S rRNA gene confirmed JCH7 and JCH3 as Streptomyces mutabilis, while JCH5 showed high genetic divergence. Overall, the results confirm the high potential of Streptomyces spp. from fallow soils as biological control agents of Botrytis sp. in blueberries.

Instituciones: Universidad Tecnológica del Perú