PLASMA ANTI-MÜLLERIAN HORMONE LEVEL AND ITS RELATIONSHIP TO OVARIAN RESERVE, SUPEROVULATION RESPONSE, AND EMBRYO SURVIVAL IN BRAHMAN (Bos indicus) FEMALES

Macias J.; Vivanco W.; Hurtado E.; Larrea C.; Andres J.; Duran P.; Hufana-Duran D. · Journal of the International Society for Southeast Asian Agricultural Sciences (2023)

Resumen

Indicators of superovulation response is needed for efficient in vivo embryo production and facilitate production of genetically superior animals. Relationships of plasma Anti-Mullerian Hormone (AMH) levels to ovarian reserve, superovulation response, and embryo survival in Brahman females were examined on January 2019 in Manabí, Ecuador. Twenty-one Brahman females were classified according to plasma AMH levels arranged in a completely randomized design with factorial treatment. Factor A corresponded to animal classification (cows and heifers), and factor B corresponded to AMH levels (100<200, 200<300, and 300<450 ρg/mL). Animals were treated with progesterone implant, estrogen benzoate injection, and intramuscular progesterone on Day 0 and a decreasing concentration of gonadotrophin on Day 4-6. Prostaglandin injection and implant removal were done on Day 6. On Day 8, gonadorelin was given and artificial insemination performed. Ovarian reserve and superovulation response were assessed by endorectal ultrasonography. Embryos were collected, quality evaluated, transferred, and pregnancy determined. Positive correlations (R2:0.73) between plasma AMH and number of ovarian reserves and number of transferable embryos were observed with increasing rates of 6.28% and 3.54%, respectively. Heifers had significantly higher corpora lutea than cows (13.7 vs 8.81, P<0.05). Embryo survival was highest in animals with higher AMH levels suggesting that selection of donors in superovulation programs should consider young animals with AMH levels above 300 ρg/mL for higher efficiency.

Instituciones: Universidad Nacional Agraria La Molina