Photoperiod-Mediated Transcriptomic Regulation of Spawning in Octopus mimus

Quispe-Pilco C.; Ferreira I.; Oliveira D.; Resende-Pinto R.; dos Santos A.G.; Delgado-Cabrera F.W.; Moreno-Salazar-Calderon K.A.B.; Fernández-Cárdenas C.P.; Tapia-Alave F.E.; Castro L.F.C.; Nande M. · Aquaculture Journal (2026) · DOI: 10.3390/aquacj6010007

Resumen

Background: The reproductive cycle of Octopus mimus is regulated by environmental and hormonal factors, with photoperiod playing a key role in spawning induction and reproductive maturation. Understanding its underlying molecular mechanisms is essential for developing strategies to enhance controlled reproduction in aquaculture. Methods: We analyzed the expression of genes involved in the photoperiod-activated spawning induction cascade in the optic lobe and its downstream effects on the oviducal gland by performing transcriptomic analyses on females exposed to continuous light (24:0), which inhibits reproductive development, and a natural photoperiod, which induces spawning. The mRNA sequencing (RNA-Seq), quality control, gene annotation, and differential expression analyses were conducted using edgeR. Results: Spawning was completely inhibited under constant light, while 80% of control females spawned. Expression profiling revealed 89 downregulated and 34 upregulated genes in the optic lobe, and 178 downregulated and 237 upregulated genes in the oviducal gland (FDR < 0.05, |log2FC| ≥ 2), including key orthologs such as FMRFamide and myomodulin. Conclusions: These results show that the optic lobe integrates photoperiodic cues that modulate reproductive activation via a neuroendocrine cascade and coordinates spawning regulation through the oviducal gland, providing insights for improving reproductive control in aquaculture systems.

Instituciones: Universidad Nacional Jorge Basadre Grohmann