Optimization of tomato (Solanum lycopersicum L.) shoot regeneration by synergistic use of adenine sulfate, glutamine and casein hydrolysate

Vargas R.; Vigo C.N.; Juarez-Contreras L.; Oliva-Cruz M. · Journal of Agriculture and Food Research (2026) · DOI: 10.1016/j.jafr.2025.102626

Resumen

Solanum lycopersicum is a widely cultivated crop, but its in vitro regeneration remains a challenging process. Although cytokinins and auxins are well known for their role in shoot regeneration, research is limited regarding the influence of other additives, such as casein hydrolysate (CH), glutamine (Gln), and adenine sulfate (AS). This work provides an optimal strategy for in vitro regeneration through the orthogonal design of Taguchi L25, a synergistic evaluation of AS, Gln, and CH, and a determination of the optimal combination. The most effective treatment (20 mg L−1 AS, 25 mg L−1 Gln, and 150 mg L−1 CH) achieved 65.63 % regeneration, significantly outperforming the control (31.25 % regeneration), resulting in an increase of more than 100 % in regeneration efficiency. On the other hand, the treatment based on 1 mg L−1 indole-3-butyric acid (IBA) and 1 mg L−1 indole-3-acetic acid (IAA) achieved an average of 8.87 roots per cutting and 100 % rooting efficiency, ensuring good survival rates during the rooting phase. The results suggest that enhanced supplementation with adenine sulfate, glutamine, and casein hydrolysate can significantly improve tissue culture efficiency potentially aiding in the optimization of cytokinins in future research. Additionally, this method offers a scalable and cost-effective alternative for tomato micropropagation, transformation, and breeding projects, potentially reducing reduce the need for high levels of cytokinins.

Instituciones: Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas