Novel approach to control sclerotium rolfsii with improved sucrose contents

Buzá M.; Lima G.; Gomes M. · Acta Microscopica (2020)

Resumen

The isolation of fungal protoplasts by means of using lytic enzymes and hypertonic media to provide osmotic support is a well-known technique to obtain cells completely deprived of cell wall. This methodology showed to be suitable for the isolation of organelles, preparation of cell-free extracts and diverse genetic purposes.

Instituciones: Universidad Científica del Sur