Nitrofuran resistance in Salmonella enterica isolated from meat for human consumption

Martínez-Puchol S.; Pons M.J.; Ruiz-Roldán L.; Laureano-Adame L.; Corujo A.; Ochoa T.J.; Ruiz J. · Revista Peruana de Medicina Experimental y Salud Publica (2020) · DOI: 10.17843/rpmesp.2020.371.4745

Resumen

The mechanisms of resistance to nitrofurans from 18 meat samples with Salmonella enterica (chicken: 15; beef: 2; pork: 1) collected in Lima (Peru) were analyzed. The isolates were serotyped and the susceptibility levels to furazolidone and nitrofurantoin [with and without the efflux pump inhibitor Phenyl-Arginine-β-naphthylamide (PAβN)], the presence of mutations in the snrA and cnr genes and the transferability of resistance by conjugation were established. Fifteen samples with S. infantis (13 from chicken samples), 2 with S. enteritidis and 1 with S. anatum were identified. All isolates except the S. anatum were resistant to both nitrofurans showing MICs (minimum inhibitory concentration) of furazolidone and nitrofurantoin of 32-64 μg/mL and 128-256 μg/mL, respectively. The addition of PAßN had no effect on the MIC levels. All nitrofuran-resistant isolates showed amino acid codon alterations at both snrA and cnr (S. infantis: snrA STOP-151; cnr STOP-137; S. enteritidis: snrA STOP-180; cnr STOP-179). No transferable mechanisms of nitrofuran resistance were detected.

Instituciones: Universidad Peruana Cayetano Heredia; Universidad Científica del Sur; Universidad Continental