Molecular characterisation of the sedentary cystoid nematode, Mesodolichodera andina (Golden, Franco, Jatala & Astogaza, 1983) gen. n., comb. n. (Tylenchida: Heteroderidae) parasitising potatoes in the Andes

Subbotin S.A.; Franco J. · Russian Journal of Nematology (2022) · DOI: 10.24412/0869-6918-2022-1-31-42

Resumen

Molecular characterisation of the sedentary cystoid nematode, Mesodolichodera andina gen. n., comb. n. parasitising potato roots collected in a potato field located in Pocsi District, Province of Arequipa, Peru is given based on the D2-D3 of 28S rRNA, ITS rRNA, COI and cytb gene sequences. This nematode was previously known as Thecavermiculatus andinus, Dolichodera andina, or Atalodera andina. Mesodolichodera andina gen. n., comb. n. was found in a mixture with Globodera rostochiensis. The phylogenetic analysis of rRNA and COI genes amplified from females of M. andina gen. n., comb. n. showed that this nematode represents an independent evolutionary lineage closely related with cyst-forming nematodes of the genera Betulodera and Vittatidera. Based on molecular results and analysis of unique morphological characters this nematode was proposed to belong to a new genus of a new subfamily under the family Heteroderidae. Atalodera spp. and Rhizonemella spp. collected from different locations in Oregon and California, USA were also molecularly characterised with several markers in this study. Phylogenetic relationships within the family Heteroderidae were reconstructed using of rRNA and COI gene sequences. © Russian Society of Nematologists, 2022.