Microbial asparaginase and its bioprocessing significance

Calderón-Toledo S.; Zavaleta A.I.; Pessoa-Junior A. · Microbial Bioreactors for Industrial Molecules (2023) · DOI: 10.1002/9781119874096.ch5

Resumen

l-Asparaginase hydrolyzes l-asparagine to l-aspartic acid and releases ammonium in a two-step process, which is used in the treatment of leukemia. In this regard, leukemic cells, unlike normal cells, possess low levels of l-asparaginase synthetase. Based on the amino acid sequences, structural homology, functionality, and sources from which they were isolated, they are divided into three families: bacterial type l-asparaginase, plant type l-asparaginase, and rhizobial type l-asparaginase. The scaling up of microbial processes is oriented toward profitable production for industrial applications, which requires implementation and control of the metabolic processes. Thus, among the most commonly used methods is batch fermentation, as applied in B. licheniformis RAM-8, which by culture at 0.75vvm and 200RPM produced 29.94U/mL of l-asparaginase at 15 hours.

Instituciones: Universidad Nacional Mayor de San Marcos