Klingler's dissection in neuroanatomy learning: narrative review with a systematic search

Laguna Y.G.; Orosco I.B.P.; Virto-Farfan H. · Revista de Neuro-Psiquiatria (2024) · DOI: 10.20453/rnp.v87i2.5252

Resumen

The Klingler dissection technique is a modified brain fixation and dissection strategy that uses freezing and thawing to reveal white matter tracts. It has been widely employed in brain anatomical investigations, allowing a better understanding of the cerebral hemispheres' complex organization. It is notable for its usefulness in the separation of white and gray matters, facilitating the dissection of fiber tracts and providing a three-dimensional understanding of the architecture of white matter systems. Objective: Examine the historical evolution, protocols, and educational, medical, and surgical applications of the Klingler dissection technique. Method: A literature review of academic articles on the topic was conducted through the exploration of databases such as SciELO, Scopus, Medline, and Google Scholar. A total of 3,521 articles of interest were identified, of which 53 were selected for evaluation. Finally 22 serving to assess the Klingler dissection protocol. Results: We addressed the historical background, application, and protocol of the Klingler method, along with its integration with technological innovations. The use of the technique in surgical approaches and post-application histological findings was also analyzed, highlighting its relevance in neuroanatomy and brain surgery. Conclusions: The Klingler white matter dissection method has been of fundamental usefulness in neuroscience, allowing for detailed anatomical descriptions, and being highly relevant in education at different academic levels.

Instituciones: Universidad Andina del Cusco