Inhibitory Effect of Remineralizing Toothpastes on the Oral Cariogenic Microbiome. In Vitro Study

Quispe-Maquera N.B.; Huayna-Yupa J.E.; Quispe-Quispe B. · Smart Innovation, Systems and Technologies (2026) · DOI: 10.1007/978-3-032-24262-4_29

Resumen

A disruption in the balance of the oral microbiome promotes the overgrowth of cariogenic bacteria, elevating the risk of dental caries. Tooth brushing and using toothpastes with antimicrobial active ingredients improve plaque removal and achieve bacterial inhibition. This study aimed to evaluate the antimicrobial inhibitory effect of four commercial toothpastes Vitis Aloe Vera (T1), Vitis Sensitive (T2), and Vitis CPC Protect (T3) as experimental groups, and Oral-B (T4) as the positive control against Streptococcus mutans and Actinomyces viscosus. Using the agar diffusion method, it was observed in S. mutans, treatment T4 exhibited the highest antimicrobial activity, closely followed by T3. Both showed greater inhibition zones compared with T1 and T2, a pattern that remained consistent at both 24 and 48 h, although with a slight reduction in the least effective treatments at 48 h. All treatment comparisons were statistically significant (p < 0.0001). In A. viscosus, T4 demonstrated the strongest growth inhibition, followed by T2, while T1 and T3 showed lower activity. Here as well, the differences between groups were highly significant (p < 0.0001). Overall, A. viscosus showed greater susceptibility than S. mutans regardless of the treatment evaluated. These findings demonstrate formulations containing sodium fluoride and CPC provide the most sustained antimicrobial effect, remaining effective for up to 48 h. The distinct responses between S. mutans and A. viscosus indicate efficacy is strain dependent. Overall, the results highlight the clinical relevance of these formulations for improving the control of cariogenic pathogens. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2026.

Instituciones: Universidad Nacional del Altiplano