In vitro ovicidal activity of “matico”, Piper tuberculatum, against “arrebiatado”, Dysdercus peruvianus; [Atividade ovicida in vitro do “matico”, Piper tuberculatum, contra o “arrebiatado”, Dysdercus peruvianus]

Elías-Agapito Y.M.F.; Rojas-Idrogo C.; Fupuy-Chung J.A.; Delgado-Paredes G.E. · Agrociencia Uruguay (2025) · DOI: 10.31285/agro.29.1659

Resumen

Dysdercus peruvianus is a hemipteran insect known as arrebiatado or cotton stainer bug that, due to its habits and morphological characteristics, affects the quality of fiber in cotton crops, with synthetic pesticides being the first barrier to control. However, excessive use has adverse effects on health and the environment. Therefore, plant extracts represent a sustainable alternative for pest control. In this study, the in vitro ovicidal activity of “matico”, Piper tuberculatum, on D. peruvianus was evaluated, determining the ovicidal effectiveness, lethal concentration (LC50) and lethal time (LC50) of the extracts of mature spikes and in vitro plants of P. tuberculatum. The CH2Cl2:MeOH (2:1) and EtOH extracts of in vitro plants achieved a percentage of inhibition in egg hatching of 94% and 82%, respectively, compared to the CH2Cl2:MeOH (2:1) and EtOH extract from mature spikes, which achieved percentages of inhibition in egg hatching of 76% and 60%, respectively. After 24 hours of evaluation, LC50 of mature spikes extracts was 0.305 and 0.351 mg/2 µL, while for the in vitro plants it was 0.229 and 0.127 mg/2 µL, respectively. The LC50 of mature spikes extract CH2Cl2:MeOH (2:1) was 24 hours, while for the in vitro plants it was 18 hours. Our study contributes to the development of more sustainable and effective alternatives for controlling D. peruvianus, reducing the dependence on synthetic pesticides and minimizing their environmental and health impacts.

Instituciones: Universidad Nacional Pedro Ruiz Gallo