IN VITRO ANTIOXIDANT AND ANTIINFLAMMATORY ACTIVITIES AND TOTAL POLYPHENOL AND FLAVONOID CONTENTS OF Anadenanthera colubrina FROM NORTHERN PERU

Samame-Caramutti E.A.; Salcedo-Valdez L.; Suárez-Cunza S. · Revista Peruana de Medicina Experimental y Salud Publica (2026) · DOI: 10.17843/rpmesp.2026.431.15207

Resumen

Objectives. To evaluate the in vitro antioxidant and anti-inflammatory activity of the hydroethanolic extract of the bark of Anadenanthera colubrina from northern Peru, as well as its total polyphenol (TPC) and flavonoid (TFC) contents. Materials and methods. Antioxidant activity was evaluated using chemical assays for 2,2-diphenyl-1-picrylhydrazyl (DPPH●) radical scavenging and ferric reducing antioxidant power (FRAP), as well as by measuring lipid peroxidation (formation of thiobarbituric acid reactive substances, TBARS) and H2 O2-induced hemolysis. Anti-inflammatory activity was determined through heat-induced ovalbumin denaturation and hypotonicity-induced hemolysis assays. Results. The extract showed high antioxidant activity in both the DPPH● radical scavenging assay (747.6 mg Trolox equivalents/gram of dry extract) and the FRAP assay (435.9 mg FeSO4 equivalents/gram of dry extract). The extract (50 to 1000 µg/ mL) inhibited lipid peroxidation, H2 O2-induced hemolysis, and ovalbumin denaturation. No activity was observed in the hypotonicity-induced hemolysis assay. TPC and TFC were 642.7 mg gallic acid equivalents/ gram and 416.2 mg catechin equivalents/gram of dry extract, respectively. Conclusions. These activities were reported for the first time for A. colubrina from northern Peru, suggesting that the hydroethanolic extract of A. colubrina bark possesses significant preliminary in vitro antioxidant and anti-inflammatory properties and that the high polyphenol and flavonoid contents may be responsible, at least partially, for these activities.

Instituciones: Universidad Nacional Mayor de San Marcos