Identification of Fungi Causing Root Rot in Oregano Crops in Southern Peru: Morphological and Molecular Analysis

Quispe-Mamani R.A.; Sulca-Quispe L.; Huanca-Mamani W.; Garcia-Castillo M.G.; Muñoz-Torres P.; Sepúlveda-Chavera G. · Pathogens (2025) · DOI: 10.3390/pathogens14080746

Resumen

Oregano (Origanum vulgare) cultivation is of great economic importance in Peru. Tacna stands out as its main producer. However, the presence of phytopathogenic fungi represents a challenge for its production. This study aimed to characterize both the morphological and molecular levels of the causal agent of crown and root rot in a crop field in the Camilaca district, Candarave, Tacna. To this end, systematic sampling was carried out using the five-gold method, collecting plants with typical symptoms. Fungi were isolated from diseased roots and characterized using macroscopic and microscopic morphological analysis as well as sequencing and multilocus phylogenetic analysis (ITS, 28S, HIS3, TEF1, TUB2). In addition, pathogenicity tests were performed on healthy plants to confirm the infectivity of the isolates. The results demonstrated that root rot was caused by a complex of phytopathogenic fungi through phylogenetic analysis of Dactylonectria torresensis, Fusarium oxysporum, F. iranicum, and F. redolens. These findings represent the first report of these species as causal agents of oregano root rot in Peru, highlighting the need for integrated management strategies that reduce the economic impact of these diseases and contribute to the sustainability of the crop in key producing regions such as Tacna.

Instituciones: Universidad Nacional Jorge Basadre Grohmann