Histomorphometry response of the small intestine mucosa in fattening guinea pigs (Cavia porcellus) challenged with Salmonella enterica var. Typhimurium

Bezada-Quintana S.G.; Carcelén-Cáceres F.D.; López-Guerra S.; Guevara-Vásquez J.E. · Revista Cientifica de la Facultad de Veterinaria (2023) · DOI: 10.52973/rcfcv-e33203

Resumen

In order to evaluate the histomorphometric response of the mucosa of the small intestine in guinea pigs challenged with Salmonella enterica var. Typhimurium (SeVT) (2×106 CFU·0.5mL·guinea pigs-1), Fifteenday-old guinea pigs (n=40) divided at random into four treatment (T) groups: 1.- guinea pigs that received a standard feed (SF) and were challenged with SeVT (T1); 2.- guinea pigs that received a SF with zinc bacitracin (50 ppm) and were challenged with SeVT (T2); 3.- guinea pigs that received a SF and were given zinc bacitracin (50 ppm) without challenge (T3), and 4.- guinea pigs that only received a SF, without challenge (T4, Control Group). Each T consisted of 10 repetitions. At the end of the experimental phase in the eighth week, samples of duodenum, jejunum and ileum from each T group were collected and stained with hematoxylin-eosin (H-E) for histomorphometry analysis. The parameters examined included villus length (VL), villus width (AVL), Lieberkühn’s crypt depth (PC) and length/crypt ratio (LV/PC). Procured data were statistically analyzed using ANOVA and Tukey’s test. The results (mean ± SD) were significantly different at P<0.05 in all T indicating villus reduced in length, width and with a LV/PC ratio less than 2:1, observed villus up to 40 % longer in duodenum, 39 % in jejunum and 55 % in ileum in the guinea pigs that were not challenged with SeVT. It is concluded that there is a negative effect on the villus histomorphometry to infection with SeVT guinea pigs. © (2023). All Rights Reserved.

Instituciones: Universidad Nacional Mayor de San Marcos