Genetic variants and serum ApoA1 and ApoB100 levels in Peruvian pregnant women with severe preeclampsia

Pacheco-Romero J.; Acosta O.; Huerta D.; Vargas M.; Cabrera S.; Mascaro P.; Huamán M.; Sandoval J.; Mateus J.; Gil E.; Guevara E.; Fujita R. · Revista Peruana de Ginecologia y Obstetricia (2024) · DOI: 10.31403/rpgo.v70i2671

Resumen

Background: Preeclampsia is a multi-organ disease that causes maternal and perinatal morbidity and mortality, with an unclear pathophysiology and without specifc prevention methods and/or treatments. Objective: To establish the association between variants -75 G/A in the ApoA1 gene, 2488 C/T in the ApoB100 gene, serum levels of ApoA1 and ApoB100 and severe preeclampsia in Peruvian pregnant women. Methods: Blood samples from pregnant women with severe preeclampsia and healthy women (controls) were processed for DNA extraction and genotyping with the PCR-RFLP technique. In addition, serum was analyzed to quantify ApoA1 and ApoB100. Genotypes and alleles were compared between cases and controls, as well as serum ApoA1 and ApoB100 levels considering genotypes. Results: No signifcant diferences were found between the genotypic and allele frequencies of the -75 G/A variant in the ApoA1 gene and 2488 C/T in the ApoB100 gene of both groups. A signifcant diference (p=0.039) was found when comparing serum ApoA1 in patients with heterozygous GA genotypes, with lower average levels in severe preeclamptic (161.37 ± 40.21) compared to controls (185.37 ± 35.38). Conclusions: Although no association was found between the variants -75 G/A variants in the APOA1 gene and 2488 C/T in the ApoB100 gene and preeclampsia, however, serum ApoA1 levels were signifcantly lower in severe preeclamptic women with GA heterozygous genotype than in controls. Thus, we contribute to the understanding of preeclampsia in Peruvian pregnant women.

Instituciones: Universidad Nacional Mayor de San Marcos; Universidad de San Martín de Porres