Genetic markers for preeclampsia in peruvian women

Pacheco-Romero J.; Acosta Conchucos O.; Huerta Canales D.; Cabrera Ramos S.; Vargas Chávez M.; Mascaro Sánchez P.; Huamán Guerrero M.; Sandoval Paredes J.; López Gabriel R.; Mateus J.; Gil Guevara E.; Guevara Rios E.; Butrica Ferré N.; Catari Soto D.; Bellido Yarlequé D.; Custodio González G.; Naranjo Adonaire A. · Colombia Medica (2021) · DOI: 10.25100/cm.v52i1.4437

Resumen

Background: Preeclampsia is a multiorgan disorder associated with maternal and perinatal morbimortality. In Peru, incidence is 10% and accounts for 22% of maternal deaths. Genome and genetic epidemiological studies have found an association between preeclampsia and genetic polymorphisms. Objective: To determine the association of the vascular endothelial growth factor (VEGF) +936 C/T and +405 G/C, interleukine-6 (IL-6)-174 G/C, IL-1β-511 C/T, Apo A-1-75 G/A, Apo B-100 2488 C/T (Xbal) polymorphisms with preeclampsia in pregnant Peruvian women. Methods: Were included preeclamptic and healthy (control) pregnant women. Maternal blood samples were subjected to DNA extraction, and molecular genetic analysis was conducted using the PCR-RFLP technique and following a specific protocol for each gene. Allele and genotypic frequencies in the cases and controls were compared. Results: No association was found between the VEGF+936C/T and VEGF+405 polymorphisms and preeclampsia. The frequencies of the GG genotypes and the G allele of the-174 G/C polymorphism in the IL6 gene in preeclamptic and controls showed significant differences, with higher frequencies in cases. For the-511 C/T polymorphism of the IL-1β gene, no significant differences were found in the frequencies of TT genotypes compared with CT+CC. The genotypes and alleles of the Apo-A1-75 G/A and Apo-B100 Xbal variants showed no significant differences between cases and controls.

Instituciones: Universidad Nacional Mayor de San Marcos