Foliar Damage Thresholds Associated with Enallodiplosis discordis Infestation in Neltuma pallida Seedlings in the Tropical Dry Forest of Northern Peru

Marigorda-Castro S.; Vilchez-Estrada K.; Javier-Alva J.; Mendoza-Martínez Y.; Talledo-Ancajima D.; Pretell-Monzón K.; Diringer B.; Granda-Wong C.; Nauray-Huari W.; Cruz G. · International Journal of Plant Biology (2026) · DOI: 10.3390/ijpb17070053

Resumen

Neltuma pallida is a multi-purpose tree species of the seasonally dry tropical forests of northern Peru, where it provides essential ecological and socioeconomic functions. However, recurrent defoliation associated with the cecidomyiid gall midge Enallodiplosis discordis may compromise early seedling establishment and the success of forest restoration programs. This study evaluated the effects of larval infestation on foliar integrity and established quantitative damage thresholds in N. pallida seedlings under dry forest conditions. Insects collected from naturally infested plants were identified using an integrative taxonomic approach that combined classical morphological diagnosis with COI-based DNA barcoding obtained by Sanger sequencing. Morphological assessment assigned the defoliating dipteran to E. discordis, while BLASTn v2.17.0. analysis of the 576-bp partial COI sequence showed 92.6% identity and 100% query coverage with Cecidomyiidae records, supporting its taxonomic placement within this family. Field bioassays conducted over a 17-week period, in which 25 individual seedlings were evaluated (N = 25), revealed a strong and significant positive correlation between larval density and foliar damage percentage (r = 0.872; p < 0.001), with moderate damage levels predominating throughout the evaluation period. Despite sustained larval presence, seedlings did not reach severe damage categories, suggesting potential relative tolerance to partial defoliation under the evaluated field conditions. Temperature and relative humidity were not significantly associated with infestation intensity or foliar damage during the study period. Overall, these findings indicate that E. discordis-associated foliar damage represents a relevant, although not necessarily lethal, biotic constraint for the early regeneration of N. pallida under the field conditions assessed. The quantitative thresholds reported here provide useful criteria for dry forest restoration programs, phytosanitary monitoring, and integrated pest management strategies in the Peruvian dry forest.

Instituciones: Universidad de Piura