EFFECT OF THE BUTANOLIC EXTRACT OF Peperomia dolabriformis ON THE DNA OF HUMAN LYMPHOCYTES EVALUATED BY THE COMET ASSAY

Meléndez-Rodríguez D.E.; Zavaleta-Espejo G.G.; Blas-Cerdán W.G.; Saldaña-Jiménez J.A. · Medicina Naturista (2023)

Resumen

The purpose of this research was to evaluate the effect of butanolic extract from Peperomia dolabriformis on the DNA from human lymphocytes. The DNA damage comet assay was evaluated in human peripheral blood lymphocytes that were cultured in PB-max karyotyping medium and incubated during 24 hours at 37 °C. Phosphate buffer and 100 uM-hydrogen peroxi-de were the negative and positive controls. Treatment groups contained 0.00125%, 0.0025% and 0.01% butanolic extract, respectively. Lymphocytes embedded in 37 °C-LMP agarose were lysed and subjected to electrophoresis under alkaline con-ditions at 300 mA, 20 V for 40 minutes. Acridine orange stained slides were examined using a fluorescent microscope, and the DNA damage was analyzed by the comet score software. The 0.01%-butanolic extract damaged the DNA with 65.06% in the Tail of the comet and 26.49 in the Tail Moment.

Instituciones: Universidad Nacional de Trujillo