Effect of supplementation of an instant mixture based on cañihua, cocoa and bovine blood on the recovery of anemia in wistar rats

Paredes Ugarte W.; Viza Salas A.G.; Zaira Churata A.; Aguirre Florez L.A.; Moroco Choqueña D.; Zapana Quispe J.; Yanqui Apaza I.; Gomez Campos R.; Cossio Bolaños M. · Nutricion Clinica y Dietetica Hospitalaria (2025) · DOI: 10.12873/451paredes-ugarte

Resumen

Objective: To verify the effect of supplementation of an instant mixture based on cañihua, cocoa and bovine blood for the recovery of anemia in wistar rats. Material and methods: An experimental study (randomized) was carried out in Wistar rats with an age range of 21-22 days of age. The 24 animals were housed in cages (two per cage). All animals lived in light/dark cycles (12 h/12 h) in a constant temperature environment (24o C ± 2o C). Four experimental groups were formed: Experimental Group 1 (EG1): [Formulation 1: Cañihua 54%, cocoa 16%, bovine blood meal 30%], Experimental Group 2 (EG2): [Formulation 2: Cañihua 47%, cocoa 13%, bovine blood meal 40%], Experimental Group 3 (EG3): [Formulation 3: Iron, Quinoa Shake] and Control Group (CG): No iron supplementation. Differences between groups were determined by two-way Anova and effect size (Cohen’s d). Results: The average age of the animals was 20-21 days old. There were significant differences in three experimental groups. In GE1 Hb increased by 40% (effect size -0.87), in GE2 it increased 22% (effect size-0.79) and in GE3 it increased 23% (effect size -0.79). The CG remained with relatively stable Hb values. There were no significant differences in the three experimental groups in Hb levels (p>0.05). Conclusion: Supplementation with the instant mixture based on cañihua (54%), cocoa (16%) and bovine blood meal proved to be effective for the recovery of anemia in Wistar rats. These results suggest that this product has a high potential as a nutritional strategy to combat iron deficiency..

Instituciones: Universidad Nacional del Altiplano