Effect of Opuntia ficus-indica “Tuna” and Vaccinium corymbosum “Blueberry” extractongenotoxicdamageinducedbycyclophosphamideinMusmusculusBalb/c

Becerra-Gutiérrez L.K.; Arriaga-Deza E.V.; Loayza-Estrada C.S.; Benel-Fernández D.; Silva-Díaz H. · Gaceta Medica Boliviana (2024) · DOI: 10.47993/gmb.v47i2.824

Resumen

Objective: to evaluate the safety and preventive and reparative efficacy of cladode extracts of Opuntia ficus-indica (prickly pear) and Vaccinium corymbosum (blueberry) fruit on genotoxic damage induced by cyclophosphamide in Mus musculus Balb/c. Methods: Randomized controlled experimental study in eight groups of eight specimens each (four females and four males): negative control, positive control (cyclophosphamide), blueberry control, prickly pear control, blueberry 1 (cyclophosphamide after), blueberry 2 (cyclophosphamide before), prickly pear 1 (cyclophosphamide after) and prickly pear 2 (cyclophosphamide before). After 21 days of treatment, the micronucleated red blood cell count was evaluated as an indicator of genotoxicity. Results: it was observed that the highest micronucleated red blood cell counts were in the positive control and tuna 1 groups, with an average of 8,38 and 11,0, respectively. Likewise, the groups with the lowest counts were the negative control and the blueberry and prickly pear controls with counts below 0.38. The extracts tested did not produce genotoxicity by themselves (p<0,05). Bilberry showed preventive anti-genotoxic efficacy and to a lesser degree, reparative efficacy (p<0,05). On the other hand, prickly pear extract also showed preventive anti-genotoxic efficacy, lower compared to blueberry (p<0,05); but, it did not evidence reparative efficacy. Conclusions: it is concluded that the extracts of prickly pear cladodes and cranberry fruit, are safe and showed preventive anti-genotoxic efficacy against cyclophosphamide-induced damage in Mus musculus Balb/c; being the preventive efficacy of cranberry superior to prickly pear, and the only one that showed reparative anti-genotoxic efficacy.

Instituciones: Universidad de San Martín de Porres