Effect of carbon-nitrogen in the native Beauveria bassiana production by solid fermentation in Ecuador

Merino-Peñafiel O.; Machado J.C.; Bayas-Morejón F.; Tigre-León A.; Merino M.; Arreguín M.; Román A. · ARPN Journal of Engineering and Applied Sciences (2020)

Resumen

The purpose of the work was to produce native Beauveria bassiana conidia at the laboratory level through Solid State Fermentation (SSF), polypropylene sleeves were used as bioreactors. The pre-cooked and sterilized substrates were: "recycled" rice and wheat, supplemented with various carbon-nitrogen sources (C-N), ratio 10: 1. The experimental design was a Categorical Multi-Factor, with 20 treatments and three repetitions to determine the variable concentration of conidia/g of bioproduct; the count of conidia was in Neubauer chamber. The mean values of controls were 1.15 x 109 Conidia/g in rice and wheat 1.22 x 109 Conidia/g with C-N supplements, the highest means of B. bassiana in rice was 1.89 x 109 Conidia/g (T7); in this same order in wheat 1.90 x 109 Conidia/g (T17). The lowest means presented: T10 and T20 (between 1.05-1.06 x 109 Conidia / g), in rice and wheat with molasses-yeast, concentration 2 (5.0-0.50 g), possibly due to an antagonistic effect between Saccharomyces cerevisiae-entomopathogenic fungi (EPF) during its cultivation. The ANOVA registered significant statistical differences (*) at ≤ p 0.05 in the factors C-N, and concentration. The Multiple Range Contrast LSD-Fisher, determined that there were no (*) between any pair of means at 95.0% confidence. It is concluded: the supplementation of C-N on rice and wheat for the mass production of B. Bassiana, influenced the yield of conidia/g of the bioproduct.

Instituciones: Universidad Nacional Mayor de San Marcos