EFFECT OF BREED AND DILUENT ON KINETIC PARAMETERS OF CHILLED AND CRYOPRESERVED RAM SEMEN UNDER CONDITIONS OF PERUVIAN HIGHLANDS

Catacora N.; García W.; Choque W.; Rodriguez F.; Condori E.; Luque N. · Spermova (2022) · DOI: 10.18548/aspe/0010.27

Resumen

The cryo-sensitivity of the ram allows us to further investigate kinetic parameters to improve cryopreservation protocols using commercially available diluents. An investigation was carried out with the objective of evaluating the kinetic parameters of chilled and cryopreserved semen from Criollo and Corriedale rams using three diluents under conditions of the Peruvian highlands. Four rams suitable for reproduction of each breed were used. Semen collection was performed four times from each ram with an artificial vagina and during the reproductive season. Semen evaluation was performed after collection and three diluents were used: Andromed, Triladyl, and Tris-egg yolk. The diluted samples were aspirated into 0.25 mL straws, slowly cooled to a temperature of 5°C and, with an equilibration time of 2 hours, cryopreservation in liquid nitrogen vapors was performed. Kinetic parameters (total motility, progressive motility, curvilinear velocity, rectilinear velocity, mean velocity, straightness, linearity, ALH and BCF) were evaluated in chilled and post-thaw cryopreserved semen using computer-assisted sperm analysis (CASA). Higher values of total motility and progressive motility of chilled semen were observed for Triladyl diluent with 92.31% and 89.31%. Although the highest values for rectilinear velocity and mean velocity were for Tris-egg yolk with 69.99 μm/s and 83.90 μm/s. For post-thaw cryopreserved semen, total motility was higher for Triladyl and Tris-egg yolk diluents with 40.61% and 33.70%, respectively. In addition, progressive motility was higher for Triladyl diluent with 33,47%. Total motility and progressive motility values were higher for chilled semen of the Criolla breed with 89.48% and 85.10%, respectively. For the post-thaw cryopreserved semen, total motility and progressive motility were also higher for the Criolla breed with 42.89% and 35.32%, respectively. The Triladyl diluent provides greater protection during refrigeration and cryopreservation of ram semen. The Criolla breed presented higher values of total motility and progressive motility in both chilled and cryopreserved semen.

Instituciones: Universidad Nacional Mayor de San Marcos; Universidad Nacional del Altiplano