Effect of a mouthwash based on propolis against Streptococcus mutans and Porphyromonas gingivalis

Vargas-Gonzales Y.; Zea-Gamboa F.; Sánchez-Tito M. · Salud Uninorte (2025) · DOI: 10.14482/sun.41.03.200.277

Resumen

Objective: To characterize chemically an ethanolic extract derived from propolis from Oxapam-pa city, Peru; Additionally, develop an experimental mouthwash based on the ethanolic extract of propolis and demonstrate its antibacterial activity against Streptococcus mutans ATCC 25175 and Porphyromonas gingivalis ATCC 33277. Materials and methods: Experimental type research, in vitro. A phytochemical march was done, followed by high-performance liquid chromatography (HPLC) for chemical analysis. Antibacterial sensitivity was evaluated by Kirby Bauer’s method and Kruskall-Wallis test followed by Dunn’s post hoc and Mann-Witney U test were used to compare the effect of propolis extract on the strains. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were de-termined. To compare the inhibition effect of the mouthwash between each strain, Student’s t-test was applied. The significance level adopted was p < 0.05. Results: Propolis from Oxapampa city, Peru, evidenced the presence of gallic acid (10.19 μg/mL) and chlorogenic acid (412.89 μg/mL). The minimum bactericidal concentration of the ethanolic extract of propolis was 30.537 mg/mL The mouthwash based on the extract formed larger inhibition halos (p<0.05) against S. mutans ATCC 25175 (29.7 mm) and P. gingivalis ATCC 33277 (28.39 mm) than 0.12% chlorhexidine (18.28 mm). Conclusions: There are differences between the antibacterial effect of the mouthwash based on ethanolic extract of propolis and 0.12% chlorhexidine against Streptococcus mutans ATCC 25175 and Porphyromonas gingivalis ATCC 33277.

Instituciones: Universidad Privada de Tacna