Differential expression of circulating micro-rnas in patients with active and latent tuberculosis

Yareta J.; Galarza M.; Capristano S.; Pellón O.; Sánchez C.; Ballon J.; Guio H. · Revista Peruana de Medicina Experimental y Salud Publica (2020) · DOI: 10.17843/rpmesp.2020.371.4468

Resumen

Objectives: To analyze the differential expression of miR-21, miR-29a, miR-99b and miR-155 in serum samples from patients with latent tuberculosis (TB) and active TB compared to healthy controls. Materials and Methods: We used 28 serum samples (9 with active TB, 10 with latent TB and 9 healthy con-trols) for the analysis of gene expression by RT-qPCR with Primers and TaqMan probes. The differential expression was calculated by the Livak method using a normalizing gene (RNU-48). Results: Overex-pression of miR-155 was found in people with latent tuberculosis, compared to healthy controls (0.63 vs. 0.01; p value = 0.032). Conclusion: The miR-155 could be considered a biomarker to differentiate latent TB from active disease. Studies with larger sample sizes are required to corroborate the findings.

Instituciones: Universidad Nacional de San Agustín de Arequipa