DIAGNOSTIC PERFORMANCE OF Leishmania braziliensis AND Leishmania peruviana ANTIGENS IN THE IMMUNOBLOT METHOD FOR THE DETECTION OF AMERICAN TEGUMENTARY LEISHMANIASIS

Rojas-Palomino N.; Sandoval-Juarez A.; Solis-Sánchez G.; Minaya-Gómez G. · Revista Peruana de Medicina Experimental y Salud Publica (2024) · DOI: 10.17843/rpmesp.2024.413.13231

Resumen

This study aimed to determine the performance of Leishmania braziliensis and Leishmania peruviana antigens in the detection of ATL by using serum samples obtained between 2013-2016. The obtained soluble and excretion/secretion antigens were transferred to membrane nitrocellulose by immunoblot assay. The evaluation was carried out against sera confirmed for ATL, at a confidence level of 95%, determining that the soluble antigen of Leishmania braziliensis had a sensitivity of 87.7%, specificity of 100% and area under the curve of 0.95; on the other hand, Leishmania peruviana showed values of 92.3%, 95.7% and 0.94, respectively. According to the results, we recommend that the reported immunogenic regions should be characterized and analyzed in order to continue with the development of recombinant and synthetic proteins, aimed at improving the efficiency of the serological diagnosis of the disease.