Detection of Patotypes of Escherichia coli Strains Causing Childhood Diarrhea in Health Establishments of the Lambayeque-Peru Region, 2018

Yacarini-Martínez A.E.; Arriaga-Deza E.V.; Beltrán-Orbegoso R.A. · Revista del Cuerpo Medico Hospital Nacional Almanzor Aguinaga Asenjo (2020) · DOI: 10.35434/rcmhnaaa.2020.133.741

Resumen

Objetive. To identify pathotypes of virulence genes of Escherichia coli from diarrheal samples of children under five years of age in the Lambayeque Region, in the period April-September 2018. The study: Descriptive and cross-sectional study. 75 E. coli strains from diarrheogenic samples were analyzed using real-time Multiplex PCR and primers for E. coli pathotype genes: daaD, aggR, eaeA, stx, ipaH and st. Findings: 34.66% (26/75) of virulence genes were detected from the total isolates. The enterotoxigenic E. coli pathotype presented the highest frequency, 18.67% (14/75) of the total isolates. It was also detected that the enterotoxigenic E. coli pathotype appeared in 53.86% (14/26) of the total positive strains. Also, in 11.54% of positive strains (3/26) they presented more than one pathotype. Conclusion: Molecular epidemiology will help a better, adequate and timely diagnosis of childhood diarrhea in this vulnerable population.

Instituciones: Universidad Nacional de Trujillo; Universidad de San Martín de Porres; Universidad Católica Santo Toribio de Mogrovejo