Detection of Mycobacterium Tuberculosis DNA in Buccal Swab Samples from Children in Lima, Peru

Flores J.A.; Calderón R.; Mesman A.W.; Soto M.; Coit J.; Aliaga J.; Mendoza M.; Leon S.R.; Konda K.; Mestanza F.M.; Mendoza C.J.; Lecca L.; Murray M.B.; Holmberg R.C.; Pollock N.R.; Franke M.F. · Pediatric Infectious Disease Journal (2020) · DOI: 10.1097/inf.0000000000002828

Resumen

We examined Mycobacterium tuberculosis DNA detection from buccal swab samples collected from children in Lima, Peru. DNA was extracted and amplified via real-time polymerase chain reaction. Sensitivity was 21% (95% confidence interval [CI]: 7%-42%) in 24 culture-confirmed tuberculosis cases and 4.6% (95% CI: 1%-13%) in 65 clinically diagnosed unconfirmed cases. Sensitivity was highest for smear-positive tuberculosis. Specificity was 99% in the 199 controls (95% CI: 96%-100%).

Instituciones: Universidad Peruana Cayetano Heredia; Universidad Privada San Juan Bautista