DETECTION OF HUMAN PAPILLOMA VIRUS IN BREAST CANCER BIOPSIES USING PCR AND IMMUNOHISTOCHEMISTRY AT THE SANTA ROSA HOSPITAL DURING 2019

Mamani-Zapana E.; Vilcapoma-Diaz M.; Ortiz-Muchotrigo N. · Revista Peruana de Medicina Experimental y Salud Publica (2022) · DOI: 10.17843/rpmesp.2022.394.11069

Resumen

The aim of this study was to determine the presence of Human Papillomavirus (HPV) type 16 and 18 in biopsies of paraffin-embedded breast tissue from patients with clinically diagnosed breast cancer. 32 paraffin-embedded breast cancer biopsies were analyzed in order to detect HPV DNA by real-time PCR, the primers were directed at the E6 gene. The histological type, histological grade and overexpression of C-erB2 and Ki-67 were evaluated by immunohistochemistry. 84.38% (27) of the samples were positive for HPV, 25% (8) were positive for HPV-16 and 59.38% (19) were positive for HPV-18. Mixed infection was found in 15.63% (5) of the samples. Overexpression of C-erbB2 and Ki-67 was seen in 6.25% (2) of the samples positive for HPV-16 and in 15.63% (5) samples positive for HPV-18. HPV-16 and HPV-18 DNA was detected in the biopsy samples analyzed by real-time PCR.

Instituciones: Universidad Nacional Mayor de San Marcos