Detection of antimicrobial resistance genes in isolates of Escherichia coli from production pigs with diarrheal diseases

Escalante D.E.; Montalvo K.A.; Alvarez L.V.; Surco R.L.; Palomino-Farfán J.; Calle S.E.; Siuce J.M. · Revista de Investigaciones Veterinarias del Peru (2022) · DOI: 10.15381/rivep.v33i5.23795

Resumen

The aim of this study was to detect antibiotic resistance genes in Escherichia coli isolates obtained from pigs with diarrhoea in intensive pig farms in Metropolitan Lima from 2017 to 2020. In total, 119 E. coli isolates were evaluated. The isolates were reactivated to extract the DNA and detect the resistance genes to the main antibiotics and of greater importance in the pig industry, such as tetracyclines (tetA, tetB and tetC), sulfonamides (sul1, sul2 and sul3), streptomycin-spectinomycin (strA/strB, aadA) and apramycin (aac(3)IV) by conventional Polymerase Chain Reaction (PCR). Results showed that 98.3% (117/119) of the isolates were positive for at least one antimicrobial resistance gene, especially the tetracycline group (88.2%). Of the 10 antimicrobial resistance genes considered, the tetA gene had the highest frequency (68%; 81/119), followed by the sul3 gene (64.7%; 77/119). The high percentage of antimicrobial resistance genes indicates a reality of the problem of bacterial resistance against antimicrobials in the study area, hence the importance of antimicrobial resistance surveillance and the correct use of antibiotics. ©Los autores. Este artículo es publicado por la Rev Inv Vet Perú de la Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos.

Instituciones: Universidad Nacional Mayor de San Marcos