CRISPR-Cas13d Induces Efficient mRNA Knockdown in Animal Embryos

Kushawah G.; Hernandez-Huertas L.; Abugattas-Nuñez del Prado J.; Martinez-Morales J.R.; DeVore M.L.; Hassan H.; Moreno-Sanchez I.; Tomas-Gallardo L.; Diaz-Moscoso A.; Monges D.E.; Guelfo J.R.; Theune W.C.; Brannan E.O.; Wang W.; Corbin T.J.; Moran A.M.; Sánchez Alvarado A.; Málaga-Trillo E.; Takacs C.M.; Bazzini A.A.; Moreno-Mateos M.A. · Developmental Cell (2020) · DOI: 10.1016/j.devcel.2020.07.013

Resumen

The development of mRNA knockdown technologies for use in vertebrate organisms such as zebrafish has been limited. Kushawah et al. establish CRISPR-RfxCas13d as an efficient, specific, cost-effective, and straightforward method for the systematic and tractable study of gene function in vivo during embryogenesis across a range of animal species.

Instituciones: Universidad Peruana Cayetano Heredia