Comparison of two isothermal methods for SARS-COV-2 screening in Callao, Peru

Cassa-Loaiza W.; Vásquez-Velásquez C.; Gonzales G.F.; Velasquez-Medrano C.; Fernandez-Delgado K.; Alvarez-Huambachano K.; Gamboa-Serpa H.; Fano-Sizgorich D. · Acta Medica Peruana (2022) · DOI: 10.35663/amp.2022.394.2475

Resumen

Objective: To compare the isothermal molecular screening techniques CPA and RT-LAMP, against the gold standard test, reverse transcription quantitative polymerase chain reaction (RT-qPCR), and to determine its agreement. Materials and Methods: Paired comparative case-control study. For the evaluation of the CPA method, 70 cases and 130 controls were used, while for RT-LAMP, 30 cases and 70 controls were used. The sensitivity and specificity of both tests were calculated. Subsequently, the bias-corrected Kappa index was calculated by resampling. Results: Both techniques have adequate and equivalent values of sensitivity (RT-LAMP: 82.8%, CPA: 83%) and specificity (RT-LAMP and CPA: 91.5%), as well as a high concordance (88%), and Kappa-index (0.72). Conclusion: Both isothermal molecular screening techniques are suitable for SARS-CoV-2 screening, with a similar sensitivity and specificity.

Instituciones: Universidad Peruana Cayetano Heredia; Universidad Privada San Juan Bautista