Chitosan-gelatin hydrogel incorporating polyvinyl alcohol and MnFe double-layered hydroxide nanocomposites with biological activity

Almajidi Y.Q.; Abdullaev S.S.; Alani B.G.; Saleh E.A.M.; Ahmad I.; Ramadan M.F.; Al-Hasnawi S.S.; Romero-Parra R.M. · International Journal of Biological Macromolecules (2023) · DOI: 10.1016/j.ijbiomac.2023.125566

Resumen

In this research, a novel nanocomposite scaffold was developed based on a natural chitosan-gelatin (CS-Ge) hydrogel by incorporating synthetic polyvinyl alcohol (PVA) and MnFe layered double hydroxides (LDHs). The CS-Ge/PVP/MnFe LDH nanocomposite hydrogels was characterized using Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Field Emission Scanning Electron Microscope (FE-SEM), Energy Dispersive X-Ray (EDX), vibrating-sample magnetometer (VSM), and Thermal gravimetric analysis (TGA). The biological tests conducted showed cell viability of the healthy cell line exceeding 95 % after 48 and 72 h. Additionally, the nanocomposite demonstrated high antibacterial activity against P. aeruginosa bacteria biofilm, as confirmed through Anti-biofilm assays. Furthermore, mechanical tests revealed that the storage modulus was greater than the loss modulus (G'/G" > 1), confirming the appropriate elastic state of the nanocomposite.

Instituciones: Universidad Continental