CARBAPENEMS AND COLISTIN RESISTANCE GENES ISOLATED IN Musca domestica FROM A GARBAGE DUMP NEAR A HOSPITAL IN LIMA

Alarcón-Calle M.A.; Osorio-Guevara V.L.; Salas-Asencios R.; Yareta J.; Marcos-Carbajal P.; Rodrigo-Rojas M.E. · Revista Peruana de Medicina Experimental y Salud Publica (2024) · DOI: 10.17843/rpmesp.2024.412.13257

Resumen

The objective was to determine the presence of carbapenem resistance genes and plasmid resistance to colistin (mcr-1) in bacteria isolated from Musca domestica in a garbage dump near a hospital in Lima, Peru. Bacteria with phenotypic resistance to carbapenemics were isolated on CHROMagar mSuperCARBATM medium and colistin resistance profiling was performed using the colistin disk elution method. Detection of blaKPC, blaNDM, blaIMP, blaOXA-48, blaVIM and mcr-1 genes was performed by conventional PCR. The antimicro-bial susceptibility profile was determined using the automated MicroScan system. We found that 31/38 strains had phenotypic resistance to carbapenemics and 26/38 strains had phenotypic resistance to colistin with a minimum inhibitory concentration ≥ 4 µg/ml. Finally, we identified seven bacterial strains with carbapenem resistance genes (OXA-48 and KPC) and one bacterial strain with plasmid resistance to colistin (mcr-1). One Escherichia coli strain had three resistance genes: KPC, OXA-48 and mcr-1.

Instituciones: Universidad Ricardo Palma; Universidad Nacional Federico Villarreal; Universidad Peruana Unión