Biotechnological and Genomic Applications in the Conservation of Native Blueberries in Natural Habitats

Arista-Fernández H.S.; Hernández-Amasifuen A.D.; Pineda-Lázaro A.J.; Guerrero-Abad J.C. · International Journal of Plant Biology (2025) · DOI: 10.3390/ijpb16030109

Resumen

The conservation of native blueberries (Vaccinium spp.) from Andean and Amazonian ecosystems faces challenges from climate change, habitat fragmentation, and land use. In this context, this review article provides a comprehensive analysis of the most relevant biotechnological and genomic tools applied to the preservation of these plant genetic resources, as well as their characterization. Among the biotechnological strategies, in vitro micropropagation delivers clonal pathogen-free valuable plants, while cryopreservation offers a viable option for a long-term germplasm storage. We also summarize its protocols focus on high regeneration rates and reproducibility. In the genomic field, we show advances in the use of molecular markers (such as SNPs, SSRs, and RAPDs), DNA barcoding and next-generation sequencing that leads genetic diversity assessment and identification of species. Finally, future perspectives in native blueberry conservation are discussed that allow the integration of emerging technologies such as landscape genomics, environmental transcriptomics, and the use of artificial intelligence tools. Integrating these approaches with the active participation of local communities can substantially strengthen the sustainable conservation of native blueberries in their natural habitats.

Instituciones: Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas