Bactericidal activity of the aqueous silver solution on enterobacteral producing extended-spectrum betalactamases

López-Esquen F.; López C.S.C.; Carrasco-Solano F.; Vergara-Espinoza M.; Ruiz-Barrueto M. · Revista del Cuerpo Medico Hospital Nacional Almanzor Aguinaga Asenjo (2023) · DOI: 10.35434/rcmhnaaa.2023.164.1953

Resumen

Introduction: Silver has been used since ancient times for its significant antibacterial potential that can be an effective alternative to combat antibiotic-resistant bacteria.. Objective: The objective was to evaluate the bactericidal activity of aqueous silver solution on strains of Escherichia coli and Klebsiella pneumoniae producing extended spectrum betalactamases (ESBL). Material and Methods: Experimental study with increasing stimulus, where 225 units of analysis were evaluated, consisting of 14 cultures of E. coli BLEE, 01 culture of Kl. pneumoniae BLEE, 5 concentrations of aqueous solution, with three applications per experiment. The electrochemical method was used to obtain the aqueous silver solution and its bactericidal activity was determined with the minimum bactericidal concentration. In addition, the growth curve of the bacterial strains was established by counting the number of bacterial colonies after exposure to the aqueous silver solution (0, 2, 4, 6, 8 and 24 hours of exposure). Results: The _MIC and BMC were 2.5 and 5mg/L for E. coli and K. pneumoniae BLEE, respectively. The concentrations of 5mg/L and 10mg/L exerted bactericidal activity against the strains evaluated, demonstrating the showing absence of bacterial growth in vitro after 4 and 8 hours of exposure, respectively. Conclusion: The aqueous silver solution presented bactericidal activity against Escherichia coli and Klebsiella pneumoniae BLEE strains, and it was observed that such activity increased with higher concentration and exposure time.

Instituciones: Universidad Nacional Pedro Ruiz Gallo; Universidad Nacional de Jaén; Universidad César Vallejo