Applications of microarrays for mass spectrometry (MAMS) in single-cell metabolomics

Ibáñez A.J.; Svatos A. · Methods in Molecular Biology (2020) · DOI: 10.1007/978-1-4939-9831-9_6

Resumen

The metabolic network is the endpoint in the flow of information that begins with the “gene” and ends with “phenotype” (observable function) of the cell. Previously, due to the variety of metabolites analyzed inside cells, the metabolomic measurements were performed with samples including multiple cells. Unfortunately, this sampling process may mask important metabolic phenomena, such as cell-to-cell heterogeneity. For these studies, we must use analytical techniques that can robustly deliver reproducible results with single-cell sensitivity. In this chapter, we summarize laser-based methods for single-cell analysis and a novel approach of MicroArrays for Mass Spectrometry (or MAMS) is described in full detail. This particular type of microarrays was tailored for the study of cells grown in liquid medium using multiple-analytical read-outs, such as optical and laser desorption/ionization (LDI) or MALDI mass spectrometry. © Springer Science+Business Media, LLC, part of Springer Nature 2020.

Instituciones: Pontificia Universidad Católica del Perú