Antibacterial effect of two hydroalcoholic extracts of medicinal plants on Streptococcus mutans

Vilchez Cáceda H.A.; Rojas Berastein K.; Olortegui Quispe A.R.; Alvia Saldarriaga C.A. · Revista Cubana de Medicina Militar (2023)

Resumen

Introduction: Hyptis obtusiflora C. Presl ex Benth (ollamepan) and Himatanthus tarapotensis (K. Schum. ex Markgr.) Plumel (bellaco caspi) present secondary metabolites with the capacity to inhibit strains of Streptococcus mutans, responsible for the development of dental caries. Objectives: To evaluate the antibacterial effect of hydroalcoholic extracts of ollamepan and bellaco caspi on Streptococcus mutans. Methods: Experimental, in vitro and comparative study. A preliminary phytochemical assay of both extracts was performed. Sixty Müller-Hinton agar plates (Merck®) were used, divided into 4 groups (n= 15): group I (70% ethanol), group II (0.12% chlorhexidine), group III (25% ollamepan), group IV (25% bellaco caspi). The disc diffusion technique described by Bauer and Kirby was used; the strain used was Streptococcus mutans ATCC 25175 and the inhibition diameters were measured at 24 h to determine the antibacterial effect. Results: In the phytochemical assay, phenolic compounds and tannins were detected in both extracts. The antibacterial effect of group III was confirmed with 18.660 ± 0.0948 mm (95.54%) and group IV with 19.383 ± 0.0845 mm (99.24%), comparable with 0.12% chlorhexidine (group II) 19.532 ± 0.0975 mm (100%) on Streptococcus mutans ATCC 25175. Conclusions: Hydroalcoholic extracts of both species have antibacterial effect in vitro on Streptococcus mutans ATCC 25175 strains. However, Himatanthus tarapotensis (K. Schum. ex Markgr.) Plumel at 25% shows antibacterial effect with values similar to chlorhexidine at 0.12%.

Instituciones: Universidad Científica del Sur; Universidad María Auxiliadora