Antibacterial activity of an experimental eucalyptus globulus labill gel against porphyromonas gingivalis

Tito M.S.; Hanco J.A. · Revista Cubana de Investigaciones Biomedicas (2021)

Resumen

Introduction: Medicinal plants have proved to have antibacterial properties for the control of periodontitis. Objective: Determine the antibacterial activity against Porphyromonas gingivalis ATCC 33277 of an experimental gel composed of essential Eucalyptus globulus Labill oil. Methods: An in vitro experimental study was conducted. The software EPi Info™ was used to estimate the repetitions. The essential oil was obtained by steam entrainment, and its chemical composition was determined by gas chromatography / mass spectrometry. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were also evaluated. An agar diffusion test was performed to measure the inhibition haloes of the 4.46% experimental gel against P. gingivalis. Comparison with 0.12% chlorhexidine was evaluated with the Mann-Whitney U test. A 5% significance level was adopted. Results: A total 11 constituents were identified in the essential oil. The main chemical components were 3-Heptadecene, (Z)-(36.13%), 1-Tridecene (14.7%) and 1,8-cineole (9.72%). MIC of the essential oil was 36.195 mg/ml, whereas MBC was 39.114 mg/ml. The inhibition haloes of the experimental P. gingivalis gel were 25.533 mm ± 0.960 mm. Statistically significant differences were observed versus 0.12% chlorhexidine (23.282 ± 0.345) (p < 0.05). Conclusions: The 4.46% experimental gel composed of Eucalyptus globulus Labill essential oil displayed considerable antibacterial activity against Porphyromonas gingivalis ATCC 33277.

Instituciones: Universidad Privada de Tacna